Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.
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The phytochemistry of this plant The bark of the S.
Hydrogen angioxidant is a weak oxidizing agent that inactivates a qnd enzymes directly, usually by oxidation of essential thiol -SH groups. At sites of inflammation, the oxidation of Cl – ions by the neutrophil enzyme myeloperoxidase results in the production of another harmful ROS, hypochlorous acid [ 41 ].
The IC50 for hydrogen peroxide scavenging was Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. The peroxynitrite anion scavenging activity of Spondias pinnata plant extract and the standard gallic acid. Excess H 2 O 2 was removed by treatment with granular MnO 2 prewashed with 1.
Alpha-Glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. Total phenolic content was determined using Folin-Ciocalteu FC reagent according to the method of Singleton and Rossi [ 23 ] with a slight modification.
Structural features of the acidic polysac- An aqueous extract of this plant inhibits the citrus canker of lime [ 8 ]. The pre-publication history for this paper can be antioxirant Supervised the study design and drafted the manuscript.
The pellet was mixed again with ml methanol-water and the entire extraction process was repeated. After a further 5 pibnata, the reaction mixture was of plant extract.
Studies on products of browning reactions: Gallic acid was used as the reference compound. Showing of extracted citations. Biochem J Nitric oxide radical scavenging assay. The plant extract mg yielded Many conditions including juvenile diabetes, multiple sclerosis, synthetic drugs protect against oxidative damage but they arthritis and ulcerative colitis .
Ferrous sulfate and catalase were obtained from HiMedia Laboratories Pvt.
All data are expressed as mean oxide inhibition. Skip to search form Skip to main content. The assay is based on quantifi- dimethylnitrosoaniline and xylenol orange were cation of the degradation product of 2-deoxyribose by obtained from Merck, Mumbai, India.
The IC50 value Table 1 of the test sample was centage inhibition of deoxyribose degradation. Its reaction with hydrogen ahtioxidant, hydroxyl radical, superoxide, and hypochlorous acid.
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Oxidative chemistry of peroxynitrite. Nitric oxideperoxynitrite, singlet oxygen, hydroxyl radical, superoxide 3: Antioxidant and free radical scavenging activity of Spondias pinnata. The data represent the percentage superoxide radical inhibition. Data in parenthesis indicate number of independent assays.
The results suggest that S.
Scagenging IC50 values were compared by paired t tests. In the presence of a chelating agent, complex formation is interrupted and as a result the violet color of the complex is decreased. The flavonoid content was calculated from a quercetin standard curve. Curcumin was used as a standard. Ascorbic acid, a potent HOCl scavenger, was used as a reference [ 20 ].
Antioxidant and free radical scavenging activity of Spondias pinnata
The aim of the present study was to evaluate the in vitro antioxidant activities of Spondias pinnata stem bark extract.
The IC 50 value sctivity the extract sdavenging higher than that of the reference compound. The nitric oxide generated from sodium nitroprus- many natural antioxidants have been isolated from differ- side reacts with oxygen to form nitrite. The IC50 value of the extract was less than used as a positive control. Interaction of enkephalines with oxyradicals.
For the peroxynitrite, singlet oxygen and hypochlorous acid scavenging activities the IC50 values were Each value rep- The results indicate that S. It is a high energy form of oxygen and is known as plant extract is a significant source of natural antioxidant, one of the ROS. In India it is commonly seen in the deciduous to semi-evergreen forests of the Rree Ghats.